Behavioural responses to separation from human companion in the domestic cat: A survey-based study.

The domestic cat is known to react to social separation, though the conceptual relationship between separation-related behaviours outside of a clinical context has not been described in detail. We did an online survey on participants who had cats in their households (Nparticipants=114; Ncats=133) and asked them to evaluate the frequency of 12 behavioural elements associated with social separation from human companions on a 5- point Likert Scale. We performed two dimensionality reduction techniques (component and factor analyses) to assess whether the specified behaviours related to social separation belonged to the same axis. We found four distinct dimensions instead of one: (a) reactivity towards companion departure cues, (b) protest behaviour towards inaccessibility, (c) unusual elimination behaviour, and (d) negative responses following social separation. Our findings suggest a manifestation of different motivational states rather than a single, separation-related construct. Future studies would benefit from a careful evaluation of the separation-related behaviours in a multi-measure context to improve the accuracy of ethological classifications.

Acute and chronic stress alter behavioral laterality in dogs.

Dogs are one of the key animal species in investigating the biological mechanisms of behavioral laterality. Cerebral asymmetries are assumed to be influenced by stress, but this subject has not yet been studied in dogs. This study aims to investigate the effect of stress on laterality in dogs by using two different motor laterality tests: the Kong™ Test and a Food-Reaching Test (FRT). Motor laterality of chronically stressed (n = 28) and emotionally/physically healthy dogs (n = 32) were determined in two different environments, i.e., a home environment and a stressful open field test (OFT) environment. Physiological parameters including salivary cortisol, respiratory rate, and heart rate were measured for each dog, under both conditions. Cortisol results showed that acute stress induction by OFT was successful. A shift towards ambilaterality was detected in dogs after acute stress. Results also showed a significantly lower absolute laterality index in the chronically stressed dogs. Moreover, the direction of the first paw used in FRT was a good predictor of the general paw preference of an animal. Overall, these results provide evidence that both acute and chronic stress exposure can change behavioral asymmetries in dogs.

Development of a safety protocol for training and using SARS-CoV-2 detection dogs: A pilot study.

Medical detection dogs have potential to be used to screen asymptomatic patients in crowded areas at risk of epidemics such as the SARS-CoV-2 pandemic. However, the fact that SARS-CoV-2 detection dogs are in direct contact with infected people or materials raises important concerns due to the zoonotic potential of the virus. No study has yet recommended a safety protocol to ensure the health of SARS- CoV-2 detection dogs during training and working in public areas. This study sought to identify suitable decontamination methods to obtain nonpathogenic face mask samples while working with SARS-CoV-2 detection dogs and to investigate whether dogs were able to adapt themselves to other decontamination procedures once they were trained for a specific odor. The present study was designed as a four-phase study: (a) Method development, (b) Testing of decon- tamination methods, (c) Testing of training methodology, and (d) Real life scenario. Surgical face masks were used as scent samples. In total, 3 dogs were trained. The practical use of 3 different decontam- ination procedures (storage, heating, and UV-C light) while training SARS-CoV-2 detection dogs were tested. The dog trained for the task alerted to the samples inactivated by the storage method with a sensitivity of100 % and specificity of 98.28 %. In the last phase of this study, one dog of 2 dogs trained, alerted to the samples inactivated by the UV-C light with a sensitivity of 91.30% and specificity of 97.16% while the other dog detected the sample with a sensitivity of 96.00% and specificity of 97.65 %.

AAV1 is the optimal viral vector for optogenetic experiments in pigeons (Columba livia).

Although optogenetics has revolutionized rodent neuroscience, it is still rarely used in other model organisms as the efficiencies of viral gene transfer differ between species and comprehensive viral transduction studies are rare. However, for comparative research, birds offer valuable model organisms as they have excellent visual and cognitive capabilities. Therefore, the following study establishes optogenetics in pigeons on histological, physiological, and behavioral levels. We show that AAV1 is the most efficient viral vector in various brain regions and leads to extensive anterograde and retrograde ChR2 expression when combined with the CAG promoter. Furthermore, transient optical stimulation of ChR2 expressing cells in the entopallium decreases pigeons' contrast sensitivity during a grayscale discrimination task. This finding demonstrates causal evidence for the involvement of the entopallium in contrast perception as well as a proof of principle for optogenetics in pigeons and provides the groundwork for various other methods that rely on viral gene transfer in birds.

Prevalence of PKD1 gene mutation in cats in Turkey and pathogenesis of feline polycystic kidney disease.

Polycystic kidney disease (PKD) is one of the most common hereditary diseases in cats, with high prevalence in Persian and Persian-related cats. PKD is caused mainly by an inherited autosomal dominant (AD) mutation, and animals may be asymptomatic for years. We screened 16 cats from various breeds exhibiting a renal abnormality by ultrasound examination and genotyped them for the c.10063C>A transversion on exon 29 of the polycystin-1 (PKD1) gene, by PCR-restriction fragment length polymorphism (PCR-RFLP). Among these cats, a Siamese nuclear family of 4 cats with ancestral hereditary renal failure were screened by whole-genome sequencing (WGS) to determine novel variations in genes associated with both AD and autosomal recessive PKD in humans. During the study period, one cat died as a result of renal failure and was forwarded for autopsy. Additionally, we screened 294 cats asymptomatic for renal disease (Angora, Van, Persian, Siamese, Scottish Fold, Exotic Shorthair, British Shorthair, and mixed breeds) to determine the prevalence of the mutation in cats in Turkey. Ten of the symptomatic and 2 of the asymptomatic cats carried the heterozygous C → A transversion, indicating a prevalence of 62.5% and 0.68%, respectively. In the WGS analysis of 4 cats in the Siamese nuclear family, novel variations were determined in the fibrocystin gene (PKHD1), which was not compatible with dominant inheritance of PKD.

The relationship between problem-solving ability and laterality in cats.

The association between hemispheric asymmetries and cognitive ability is one of the key areas of comparative laterality research. In several animal species, individual limb preferences correlate with perceptual, cognitive, or motor abilities, possibly by increasing dexterity of one limb and minimizing response conflicts between hemispheres. Despite this wealth of research, the association between laterality and cognitive abilities in the cat (Felis catus) is not well understood. Therefore, it was the aim of the present study to investigate the relationship between laterality and problem-solving ability in cats. To this end, strength and direction of paw preferences in 41 cats were measured using two novel food reaching tasks in which the animals needed to open a lid in order to reach the food reward. We found that cats that showed a clear preference for one paw were able to open more lids succesfully than ambilateral animals. Moreover, cats that preferred to interact with the test apparatus with their paw from the beginning, opened more lids than cats the first tried to interact with the test apparatus using their heads. Results also suggested a predictive validity of the first paw usage for general paw usage. It was also shown that the cats' individual paw preferences were stable and task-independent. These results yield further support to the idea that lateralization may enhance cognitive abilities.

Functional cerebral asymmetry in dogs living under different environmental conditions.

Investigating the link between stress and functional cerebral asymmetry (FCA) has been a subject of interest in recent years. The demonstration of this link in the field of veterinary medicine is particularly important as measuring FCA has the potential to be an alternative and non-invasive behavioral method to assess stress in dogs. The present study aimed to investigate whether FCA is affected by different environmental conditions in dogs. The main aim of this study was to investigate the changes in FCA in dogs living under different conditions. To this aim, strength and direction of FCA in 40 urban free-ranging dogs were measured by a Kong test. Dogs were divided into four groups considering their environmental conditions: The dogs in Group 1 (n = 8) were rehabilitated urban free ranging dogs, which were kept in enriched shelter conditions. The dogs in Group 2 (n = 9) were adopted free ranging dogs, which lived in home environment for more than 1 year. The dogs in Group 3 (n = 11) were urban free ranging dogs, which stayed in individual cages in a dog shelter for more than 6 months. The dogs in Group 4 (n = 12) were adopted urban free ranging dogs, which stayed in a dog pension for more than 30 days. Considering the length of their stay in a kennel environment, quality of living condition and emotional states, the dogs in Group 3 and 4 were classified as chronically stressed dogs. Statistically significant differences existed between groups considering strength and direction of lateralization. Most of the dogs in Group 1 (87.5 %) and Group 2 (77.8 %) showed significant paw preferences, whereas most of the dogs in the Group 3 (72.7 %) and Group 4 (75 %) were categorized as ambilateral. Considering the individual level asymmetry in dogs and environmental conditions of dogs in Group 3 and 4, one may suggest that high ambilaterality levels is related with chronic stress. Thus, reduced FCA may not be the reason for stress sensibility, rather it can be an outcome of stressful situations. These results are the first to demonstrate the possible link between chronic stress and ambilaterality in dogs.

Paw preferences in cats and dogs: Meta-analysis.

Predator-prey relationships have been suggested to be one of the primary evolutionary factors driving the development of functional hemispheric asymmetries. However, lateralization in many predator species is not well understood and existing studies often are statistically underpowered due to small sample sizes and they moreover show conflicting results. Here, we statistically integrated findings on paw preferences in cats and dogs, two predator species within the Carnivora order that are commonly kept as pets in many societies around the globe. For both species, there were significantly more lateralized than non-lateralized animals. We found that 78% of cats and 68% of dogs showed either left- or right-sided paw preference. Unlike humans, neither dogs nor cats showed a rightward paw preference on the population level. For cats, but not dogs, we found a significant sex difference, with female animals having greater odds of being right-lateralized compared to male animals.